I. Product Core Positioning

β-Nicotinamide Adenine Dinucleotide, Reduced Form (NADH, CAS 606-68-8), also known as Coenzyme I (Reduced Form), is the core redox cofactor and hydrogen carrier in living organisms. As the reduced form of NAD+, it serves as the primary electron donor in mitochondrial oxidative phosphorylation, directly driving ATP synthesis.

Leveraging our proprietary enzymatic synthesis technology, we produce high-purity, high-activity NADH bulk powder. With strict control over stereochemistry and minimal impurity residues, we provide compliant and efficient raw material solutions for IV infusion therapy, anti-aging health management, preclinical research, and biomanufacturing. NADH is the critical bioactive base material for high-end applications in the biotech and healthcare industries.

II. Basic Physicochemical Parameters (Precise Detection Version)

Item	Authoritative Parameter Index	Detection Standard
Full Name	β-Nicotinamide Adenine Dinucleotide (Reduced Form)	International Nomenclature
CAS Number	606-68-8	Unique Chemical Identifier
Molecular Formula	C21​H29​N7​O14​P2​	Exact Mass: 665.45
Molecular Weight	665.45 (Precise Value)	High-Resolution Mass Spectrometry
Appearance	White or Slightly Off-White Crystalline Powder	Visual Inspection + Microscopy
Purity	≥98% (HPLC Detection)	C18 Column, 260nm Wavelength
Assay	≥95.0% (UV Absorption at 260nm)	USP/EP Reference Method
Endotoxin Content	≤0.2 EU/mg	TAL Method, EP/USP Compliant
Heavy Metals	≤0.5 ppm	Atomic Absorption Spectroscopy
Microbial Limit	TPC ≤10 CFU/g; E. coli/Salmonella Negative	Plate Count + Biochemical Identification
Solubility	Freely Soluble in Water (Clear at 5mg/mL); Insoluble in Organic Solvents	Solubility Assay
Storage Conditions	Powder: -20℃, Light-Proof, Sealed, Dry; Stable for 36 Months	Accelerated Stability (40℃/75%RH)
Packaging	1g/10g/100g/1kg/25kg; Custom Sterile Inner Bags	Food/Medical Grade Packaging

III. Core Biological Functions & Technical Application Value

1. The Primary Electron Donor: ATP Synthesis Engine

NADH is the central electron and hydrogen carrier in cellular respiration. During oxidative phosphorylation in mitochondria, NADH transfers electrons to the electron transport chain (Complex I), driving proton gradient formation and powering ATP synthase to produce >90% of the cell’s energy.

Technical Application: In IV energy infusion, exogenous NADH directly replenishes the reducing equivalent pool, enhancing mitochondrial respiration efficiency. It is particularly effective for alleviating fatigue, improving metabolic rate, and supporting post-exercise recovery. When combined with Coenzyme Q10 and Magnesium, it achieves synergistic enhancement of energy metabolism.

2. Antioxidant & Anti-Aging: Cellular Defense & Regeneration

As a potent endogenous antioxidant, NADH scavenges reactive oxygen species (ROS) and maintains the cellular redox balance. It also upregulates the activity of Sirtuins (SIRT1-7) and PARP family enzymes, promoting DNA damage repair, slow cellular senescence, and stimulating collagen synthesis in skin fibroblasts.

Technical Application: In high-end anti-aging IV therapy, NADH reduces oxidative stress, improves skin elasticity, and mitigates cognitive decline associated with neurodegeneration. It is a core component of “cell rejuvenation” protocols.

3. Neuroprotection & Cognitive Support

NADH plays a pivotal role in cerebral energy metabolism and neurotransmitter synthesis (e.g., dopamine, serotonin). It modulates NMDA receptor activity, enhances synaptic plasticity, and protects neurons from ischemic damage.

Technical Application: For neurological health management, NADH is used to support cognitive function, improve focus, and aid in the recovery of neurodegenerative conditions. It can be synergistically combined with Neuropeptides (Semax, Selank) and Thymosin β4 for enhanced neurorestorative effects.

4. Technical Application Scenarios

Application Field	Core Technical Value	Synergy Formulation
IV Infusion Therapy	High Purity & Low Endotoxin; Aseptic-Compatible	NADH + Glutathione + NR
Preclinical Research	High Activity & Batch Consistency; HPLC/LC-MS Verified	NADH + Assay Kits
Anti-Aging Health Management	Redox Regulation & Mitochondrial Support	NADH + Vitamin B Complex + Antioxidants
Formulation Development	Compatible with Freeze-Dried Powder & Injection	Bulk NADH + Sterile Excipients

IV. Our Core Technical Advantages (Differentiated Competitiveness)

1. Enzymatic Synthesis: Stereochemically Precise & Active

We employ recombinant Escherichia coli-expressed NAD+ synthetase (NadE/NadD) for the reduction of NAD+ to NADH. This enzymatic process:

• Eliminates the need for harsh chemical reagents, ensuring no chiral impurities;
• Achieves a biological activity retention rate of ≥98%, far exceeding chemical synthesis standards;
• Enables automated, continuous production with batch-to-batch purity variation ≤0.2%.

2. Advanced Purification: Ultimate Purity & Safety

Our three-stage purification system guarantees exceptional quality:

1. Membrane Separation: Nanofiltration removes macromolecular impurities and salts;
2. HPLC Refinement: Preparative chromatography isolates the active β-isomer from trace by-products;
3. Low-Temperature Crystallization: Controlled recrystallization at -5°C yields high-purity crystals with minimal water content.

3. Strict Endotoxin Control: Medical-Grade Safety

We implement a dual endotoxin removal process (activated carbon adsorption + ultrafiltration) in a Class 10,000 cleanroom. This ensures our NADH meets the strictest ≤0.2 EU/mg threshold, making it safe for parenteral administration.

4. Stability & Logistics: Global Supply Assurance

• Stabilization Technology: Vacuum drying and nitrogen-flushed packaging minimize oxidation;
• Cold-Chain Logistics: International shipments (e.g., Greece, EU) are delivered via temperature-controlled transport with dry ice, ensuring product integrity.

V. Compliance & Quality Assurance System

1. Authoritative Certifications

Our NADH complies with:

• ISO9001:2015 & ISO14001:2015;
• USP/EP Pharmacopoeia Standards;
• FDA Registration& HALAL Certification;
• EU REACH Compliant.