I. Product Core Positioning

β-Nicotinamide Adenine Dinucleotide (NAD+, CAS 53-84-9), also known as Coenzyme I (Oxidized Form), is the core redox cofactor and signal molecule hub in living organisms. It is deeply involved in hundreds of core physiological processes such as cellular energy metabolism, DNA damage repair, gene expression regulation, and mitochondrial function maintenance.

Based on our core enzymatic synthesis technology, we have developed high-activity, low-endotoxin NAD+ bulk powder. With precise molecular synthesis technology and strict quality control, we provide compliant and efficient raw material supply solutions for IV infusion therapy, high-end anti-aging health management, cutting-edge biomedical research and other fields. It is the core base material for high-end applications in the biotech industry.

II. Basic Physicochemical Parameters (Precise Detection Version)

Item	Authoritative Parameter Index	Detection Standard
Full Chinese Name	β-Nicotinamide Adenine Dinucleotide (Oxidized Form)	Pharmacopoeia/Industry Standard
Full English Name	β-Nicotinamide Adenine Dinucleotide (Oxidized Form)	International Nomenclature
CAS Number	53-84-9	Unique International Chemical Identifier
Molecular Formula	C21​H27​N7​O14​P2​	Precise Molecular Structural Characterization
Molecular Weight	663.43 (Precise Value)	Mass Spectrometry (MS) Detection and Verification
Appearance	White or Off-White Crystalline Powder	Visual Inspection + Microscopic Particle Size Analysis
Purity	≥99.5% (HPLC Detection)	High-Performance Liquid Chromatography (C18 Column, 260nm Wavelength)
Endotoxin Content	≤0.2 EU/mg	Tachypleus Amebocyte Lysate (TAL) Method, Compliant with EP/USP Standards
Heavy Metal Content	≤0.5 ppm	Atomic Absorption Spectrophotometry (AAS)
Microbial Limit	Total Viable Count ≤10 CFU/g; No Detection of Escherichia coli/Salmonella	Plate Count Method + Biochemical Identification
Solubility	Freely Soluble in Water (Clear and Transparent at 10mg/mL in Water at 25℃); Insoluble in Non-Polar Solvents Such as Ethanol, Acetone, Diethyl Ether	Solubility Determination Method
Storage Conditions	Powder: -20℃, Light-Proof, Sealed, Dry Environment, Stable for 36 Months; 4℃ Environment, Stable for 24 Months	Accelerated Stability Test (40℃/75%RH) Verification
Packaging Specification	1g/10g/100g/1kg/25kg (Customizable Packaging Supported, Sterile Inner Bags Available)	Food Grade/Medical Grade Packaging Materials Optional

III. Core Biological Functions & Technical Application Value

1. Core Molecular Engine of Cellular Energy Metabolism

As the core hydrogen transporter in the mitochondrial electron transport chain, NAD+ is an essential substrate for energy production pathways such as glycolysis, tricarboxylic acid cycle, and oxidative phosphorylation. It participates in more than 90% of intracellular ATP synthesis. The nicotinamide group in its molecular structure reversibly accepts hydrogen atoms and electrons, transferring energy through mitochondrial Complexes I and II, and significantly improving the efficiency of the cellular mitochondrial respiratory chain.

Technical Application: In IV energy infusion therapy, exogenous supplementation of NAD+ can directly supplement cellular energy metabolism substrates,chronic fatigue, slow metabolism, physical decline and other problems caused by mitochondrial function decline. Combined with raw materials such as Coenzyme Q10 and ATP, it can fully activate the energy metabolism pathway and improve the clinical effect of infusion.

2. Key Regulator of NAD+-Dependent Enzyme System

NAD+ is the sole substrate of core enzymes such as Sirtuins deacetylases, PARP polymerases, and CD38/CD157. By regulating the activity of these enzymes, it achieves precise regulation of cellular senescence, DNA repair, and inflammatory responses:

• Sirtuins Family (SIRT1-7): Regulates telomere maintenance, apoptosis, and lipid metabolism by deacetylating histones and non-histone proteins, delaying cellular senescence;
• PARP Family: Identifies and repairs single/double-strand DNA damage, maintains genome stability, and reduces cellular mutation risk;
• CD38/CD157: Regulates the NAD+ metabolic cycle, affecting immune cell activation and inflammatory factor secretion.

Technical Application: In high-end anti-aging IV therapy, NAD+ can promote collagen synthesis in skin fibroblasts and reduce free radical damage by regulating the above enzyme system, while improving cognitive decline related to neurodegenerative diseases. It is the core raw material for “cellular-level anti-aging”.

3. Signal Link for Nerve and Immune Regulation

In the central nervous system, NAD+ participates in the synthesis and signal transmission of neurotransmitters (such as glutamate and dopamine), regulating neuronal excitability by activating NMDAR receptors and improving neural synaptic plasticity; in the immune system, it maintains immune balance by regulating the activation of T cells, B cells, macrophages and cytokine secretion.

Technical Application: For nerve repair and immune regulation IV courses, NAD+ can be synergistically combined with raw materials such as neuropeptides (Semax, Selank) and Thymosin β4. Through the dual mechanism of “energy supplementation + signal regulation”, it improves nerve repair efficiency and immune cell activity, expanding the clinical application scenarios of the course.

4. Technical Application Scenarios in Segmented Fields

Application Field	Core Technical Value	Compatible Raw Material Synergy Plan
IV Infusion Therapy	High Activity & Low Endotoxin, Compatible with Aseptic Infusion Process, Improving Clinical Safety and Efficacy	NAD+ + Glutathione (GSH) + Nicotinamide Riboside (NR)
Cutting-Edge Biomedical Research	High-Purity Active Raw Materials, Supporting Research on Cellular Metabolism, DNA Repair, Enzymatic Mechanisms, etc.	Research-Grade NAD+ + Detection Kit (HPLC/LC-MS Compatible)
High-End Health Management	Customized Infusion Formulas, Suitable for Different Populations (Middle-Aged & Elderly Anti-Aging, Working Population Fatigue Regulation, etc.)	NAD+ + Amino Acid Complex Preparation + Vitamin Group
Formulation Development	Compatible with Dosage Form Development Such as Freeze-Dried Powder, Injection, Nasal Spray, Supporting Aseptic Filling Process	Bulk NAD+ + Aseptic Excipients + Filling Technical Services

IV. Our Core Technical Advantages (Differentiated Competitiveness)

1. Enzymatic Synthesis Technology: Precise Synthesis & Unimpaired Activity

Adopt biological enzymatic directed synthesis technology, using Nicotinamide Mononucleotide (NMN) and ATP as substrates, and catalyze the reaction through NAD+ synthetase (NadE/NadD) expressed by recombinant Escherichia coli to achieve precise molecular synthesis:

• Compared with traditional chemical synthesis, enzymatic synthesis does not require high temperature and pressure, has no organic solvent residue, avoids chiral impurities and chemical by-products, and the retention rate of product biological activity is ≥98%;
• Adopt immobilized enzyme technologyto realize continuous and automated production of enzymatic reaction. The purity fluctuation between batches is ≤0.2%, which is stable and suitable for large-scale mass production and customized demand.

2. Multi-Stage Purification Technology: Ultimate Purity & Compliance Standards

Achieve ultimate purification of NAD+ through a three-stage purification system, completely removing impurities and endotoxins:

1. Membrane Separation Purification: Use nanofiltration membrane to intercept impurities with molecular weight >500Da, removing small molecule metabolites and salts;
2. High-Performance Liquid Refining: Use preparative HPLC for precise separation to further remove isomers and trace impurities;
3. Low-Temperature Crystallization: Perform recrystallization in a low-temperature environment of -5℃ to improve crystal purity and stability. The final product purity is ≥99.5%, and the endotoxin content is far lower than the medical-grade raw material standard.

3. Aseptic & Stability Technology: Full-Link Guarantee

• Endotoxin Control Technology: Adopt activated carbon adsorption + ultrafiltration dual endotoxin removal process, combined with full-aseptic production environment (Class 10000 clean workshop), to ensure that the endotoxin content is ≤0.005 EU/mg, meeting the aseptic requirements of IV infusion;
• Stability Enhancement Technology: Through vacuum freeze-drying pretreatment (for small-batch customized products) and nitrogen-filled packaging process, reduce oxidation and moisture absorption risks. The purity reduction rate is <0.3% under -20℃ storage condition for 36 months;
• Cold-Chain Logistics Technology: Equipped with a professional temperature-controlled logistics system, the entire transportation process is maintained at -20℃, matched with insulation boxes and dry ice packaging, to ensure no loss of product quality during cross-border transportation (such as Greece, EU).

4. Full-Process Technical Services: One-Stop Support from Raw Materials to Applications

• Formulation Process Adaptation: Professional formulation R&D team can provide NAD+ IV infusion formula optimization solutions according to customer needs, including concentration ratio, pH value regulation (adapted to human physiological pH 7.2-7.4), compatibility stability test, assist in connecting with aseptic filling partners, and complete compliant development of dosage forms such as freeze-dried powder injections and injections;
• Multilingual Technical Documents: Provide multi-language “Product Technical Manuals” in English, Greek, etc., covering COA, purity test reports, microbial/endotoxin test data, storage specifications, infusion safety guidelines, etc., to support compliant promotion in overseas markets;
• Customized Technical Services: Support customized purity (99.5%-99.9%) and endotoxin indicators (≤0.01 EU/mg to ≤0.001 EU/mg), adapting to different application scenarios such as research-grade, medical-grade, and high-end health management.