Nicotinamide Riboside Hydrogen Malate (NRHM)

English Name: Nicotinamide Riboside Hydrogen Malate (NRHM)
CAS No.: 2415659-01-5 
Molecular Formula: C15H20N2O10
Molecular Weight: 388.33
Product Grades: Chemical Grade, Food Grade, Research Grade (Customizable as required)
Core Features: A stable crystalline compound formed by salt-type design of NR and malic acid, with stability and bioavailability far superior to ordinary NR chloride, no obvious hygroscopicity, suitable for multi-scenario applications

1. Product Overview

Nicotinamide Riboside Hydrogen Malate (NRHM) is a new type of crystalline compound formed by precise salt-type design of Nicotinamide Riboside (NR) and malic acid. It is a high-end active derivative of Vitamin B3 and an efficient precursor of NAD⁺ (Nicotinamide Adenine Dinucleotide). Essentially, it combines NR and malic acid through scientific proportioning. After entering the human body, it can quickly dissociate into two fully studied basic molecules, which participate in physiological metabolism respectively to achieve a functional synergy effect. At the same time, it solves the industry pain points of ordinary NR chloride, such as easy degradation, strong hygroscopicity and poor stability. Relying on the independently developed exclusive enzymatic synthesis process, Maintain Biotech has broken through the bottleneck of crystal form regulation technology. The produced NRHM products have the core advantages of high purity, low impurities, environmental friendliness, outstanding stability and high bioavailability. They are widely used in high-end fields such as dietary supplements, pharmaceutical intermediates and biological research, and are the upgraded choice of NAD⁺ precursor raw materials, demonstrating the technical strength and application value of core raw materials in high-end biochemical industry.

2.Core Product Efficacy

The core efficacy of Nicotinamide Riboside Hydrogen Malate (NRHM) focuses on the precise regulation of NAD⁺ levels. It combines the core physiological activity of NR and the metabolic auxiliary effect of malic acid. Compared with ordinary NR products, it has stronger stability, higher absorption efficiency, prominent safety and wider application scenarios. The details are as follows:

• Efficient Regulation of NAD⁺ Levels: As a high-quality NAD⁺ precursor, it can quickly penetrate the cell membrane and enter cells, gently and efficiently increase the level of NAD⁺ in the body, make up for the loss of NAD⁺ caused by aging and excessive pressure, provide stable support for cellular energy metabolism and DNA repair, and its effect is superior to ordinary NR chloride.
• Cell Protection and Anti-Aging: It is deeply involved in cellular DNA repair and oxidative stress regulation, efficiently scavenges free radicals in the body, reduces oxidative damage, slows down the process of cellular aging, improves cell vitality, and further enhances the anti-aging effect with the help of the antioxidant auxiliary effect of malic acid.
• Improvement of Metabolic Functions: It dual-regulates mitochondrial function, promotes fat decomposition and energy metabolism, relieves physical fatigue and improves endurance. At the same time, malic acid can assist in regulating the acid-base balance in the body, helping to improve metabolic efficiency, which is suitable for the R&D needs of sports nutrition and anti-fatigue products.
• Neuroprotective Effect: It can smoothly pass through the blood-brain barrier, provide sufficient nutrition for nerve cells, promote the synthesis of neurotransmitters, improve cognitive function, memory and concentration, and assist in the prevention of neurodegenerative injuries, which is suitable for the R&D of brain health products.
• Anti-Inflammatory, Antioxidant and Safety Adaptability: It inhibits the release of inflammatory factors, enhances the body’s own antioxidant capacity, reduces damage to tissues and organs, and improves immunity; through toxicological evaluation, high-dose administration has no obvious adverse reactions and no nicotinic acid flushing side effects, suitable for long-term application scenarios.

3.Core Process Advantages (Maintain’s Exclusive Enzymatic Crystal Form Regulation Technology)

There are three main production methods for NRHM. The enzymatic synthesis combined with crystal form regulation process independently developed by Maintain Biotech breaks through the bottleneck of traditional processes and has more advantages compared with ordinary NRHM production technologies. The details are as follows:

(1) Traditional Chemical Synthesis Method

It is prepared by chemical condensation and salification reactions using nicotinamide, ribose derivatives and malic acid as raw materials, which requires a large amount of organic solvents and strong acid-base catalysts. The disadvantages are obvious: product purity ≤95%, high impurities, easy to produce harmful residues, unstable crystal form, large emissions of three wastes, harsh reaction conditions, high cost and poor batch stability, which cannot be adapted to high-end application scenarios.

(2) Microbial Fermentation Method

NR is synthesized through microbial metabolism, and then salified with malic acid, which does not require a large amount of organic solvents, but has obvious limitations: low product concentration, yield ≤60%, long fermentation cycle (7-10 days), poor crystal form controllability, large purity fluctuation, need for additional refining, difficulty in large-scale industrial production, and insufficient product stability.

(3) Maintain’s Exclusive Enzymatic Crystal Form Regulation Process (Recommended)

Adopting the exclusive dual-enzyme one-pot method + precise crystal form regulation technology, using nicotinamide, purine nucleosides and malic acid as substrates, in a mild reaction system of normal temperature and pressure and full aqueous phase, NRHM with specific crystal structure is synthesized by strictly regulating temperature, cooling rate and crystallization conditions. Compared with traditional processes, it has irreplaceable core advantages and can realize multi-dimensional customized production:

• Environmental Friendliness: No organic solvents are added throughout the process, no three wastes are emitted, which fully conforms to the development trend of the green biocatalysis industry, avoids the risk of catalyst residues, can be directly used in high-end scenarios such as food and medicine, and meets international certification standards such as FDA GRAS.
• High Purity and High Yield: The conversion rate of nicotinamide is ≥99%. After multi-stage refining and crystal form optimization, the product purity can reach up to 99.5%+, the impurity content is far lower than international standards, and the total yield is greatly improved, which significantly reduces the production cost of customers.
• Stable and Controllable Crystal Form: Through precise regulation of process parameters, crystalline products with excellent stability are obtained. Compared with ordinary NR chloride, the sensitivity to light, air and humidity is significantly reduced, the hygroscopicity is low, it is not easy to degrade or discolor during storage and transportation, and can maintain biological activity for a long time.
• Stable and Efficient Process: The reaction conditions are mild (25-30℃, pH 6.0-7.0), the whole process is traceable, the batch difference is small, suitable for large-scale industrial production, and completely solves the industry pain points of unstable crystal form and large batch fluctuation in traditional processes.
• Strong Customization: It can flexibly customize powder crystal form (α/β configuration and special crystal forms), bulk density and particle size, can be processed into pellets (controlled release/rapid release) and provide exclusive formula customization, fully meeting the personalized production needs of customers in different fields.

4.Core Product Parameters

Parameter Name	Chemical Grade	Food Grade	Research Grade
Purity (HPLC)	≥98.0%	≥99.0%	≥99.5%
Appearance	White to pale yellow crystalline powder, no caking	White crystalline powder, odorless, no caking	White needle-like crystals, no impurities, no moisture absorption
Powder Crystal Form	Conventional crystal form, customizable	Conventional crystal form, customizable (α/β configuration)	High-purity β configuration, customizable special crystal form
Bulk Density	0.45-0.65g/cm³ (Customizable)	0.55-0.75g/cm³ (Customizable)	0.60-0.80g/cm³ (Customizable)
Particle Size	80-120 mesh (Customizable 20-200 mesh)	100-150 mesh (Customizable 40-200 mesh)	120-180 mesh (Customizable 60-200 mesh)
Moisture	≤4.0%	≤2.5%	≤1.5%
Heavy Metals	≤10ppm	≤5ppm	≤1ppm
Microbial Limit	Complies with chemical grade standards	Total bacterial count ≤1000cfu/g, mold and yeast ≤100cfu/g	Sterile (Customizable as required)
Storage Conditions	Sealed and stored at -20℃, avoid light and moisture	Sealed and stored at -20℃, avoid light and moisture	Sealed and vacuum stored at -20℃
Shelf Life	12 months (sealed storage)	24 months (sealed storage)	36 months (vacuum sealed storage)

5.Core Application Areas

Relying on the core advantages of high purity, high stability and high bioavailability, combined with the functional synergy of NR and malic acid, Maintain Biotech’s NRHM is widely used in many high-end fields, accurately meeting the customized needs of different scenarios, and has more application advantages compared with ordinary NR products:

1. Dietary Supplements & Health Products Field: As an efficient NAD⁺ precursor, it can gently increase the level of NAD⁺ in the body, alleviate cell aging, improve metabolic function and brain health, has no nicotinic acid flushing side effects, and is suitable for long-term supplementation. It supports customization of crystal form, particle size, bulk density, pellet processing (controlled release/rapid release) and formula customization, and is compatible with various dosage forms such as capsules, tablets and freeze-dried powder. It is the core upgraded raw material for high-end anti-aging, brain health and sports nutrition products, has obtained FDA NDI safety certification, and is suitable for the global high-end health product market demand.
2. Pharmaceutical Intermediate Field: Used in the R&D and production of coenzyme drugs (such as NAD⁺, NADP⁺) and related drugs for neurodegenerative diseases and metabolic syndrome. According to the needs of pharmaceutical R&D, it can customize powder crystal form, particle size and bulk density, provide pellet customization and exclusive formula solutions, and provide high-adaptability raw material support for the pharmaceutical field with the advantages of high purity, high stability and no residue, helping the R&D of new drugs.
3. Biological Research Field: As a NAD⁺ precursor raw material with excellent stability, it provides reliable and stable experimental materials for scientific research experiments related to cell metabolism, aging mechanism and neuroprotection. It can customize high-purity special crystal form products according to research needs, helping the efficient development of scientific research work.
4. Customization Service Supplement: Provide full-process customization services, including but not limited to: powder crystal form (α/β configuration and special crystal forms) customization, bulk density (0.45-0.80g/cm³) customization, particle size (20-200 mesh) customization, pellet (specification, release speed) customization, and exclusive formula customization for different products, fully meeting the diverse and personalized production needs of customers, and providing one-on-one technical support and solutions.