NMN

I.Core Product Positioning

**β-Nicotinamide Mononucleotide (NMN, CAS 1094-61-7)** is a key precursor for NAD+ (Coenzyme I) synthesis in organisms, with the molecular formula C₁₁H₁₅N₂O₈P and a relative molecular mass of 334.22. It exists in two isomers, α-NMN and β-NMN, among which β-NMN is the only biologically active form. It can quickly penetrate cell membranes, replenish intracellular NAD+ levels, and regulate hundreds of core physiological processes in the human body.

Based on the industry-leading **full enzymatic synthesis process**, we produce high-purity, low-impurity, and high-activity NMN bulk powder. Strictly adhering to the standards of the Chinese Pharmacopoeia (2020 Edition, Volume IV) and USP/EP international pharmacopoeial specifications, our products feature strong stability in mass production. They are widely applicable to IV infusion therapy, high-end anti-aging health management, cutting-edge biological research, and dietary supplement formulation R&D. We provide compliant, efficient, and stable core raw material support for the biotechnology and health care industries. As one of the most widely used and cost-effective NAD+ precursor raw materials currently available, NMN helps partners seize opportunities in the high-end raw material market.

II.Basic Physicochemical Parameters (Precision Detection Version)

III. Core Biological Functions and Technical Application Value

1.Efficient NAD+ Supplementation: The “Reversal Switch” for Cellular Aging

As the most direct precursor of NAD+, NMN can directly penetrate cell membranes into cells without multiple metabolic conversions, rapidly increasing intracellular NAD+ levels. The NAD+ content in the human body in middle age is only 50% of that in young age, and supplementing NMN can effectively reverse this decline process, activate cellular energy metabolism and repair mechanisms, and delay cellular aging from the root. It is the core raw material support for “cellular-level anti-aging”, a mechanism that has been confirmed by many international scientific research studies.

Technical Application: In high-end anti-aging IV infusion therapy, NMN can directly supplement cellular NAD+ reserves, and when combined with raw materials such as Glutathione (GSH) and Coenzyme Q10, it can synergistically enhance anti-aging effects. In the field of dietary supplements, it can be made into enteric-coated capsules, oral solutions and other dosage forms to improve bioavailability, adapting to the needs of the general public for anti-aging and staying up late conditioning.

2.Core Engine of Cellular Energy Metabolism

NAD+ is a key coenzyme in the mitochondrial oxidative phosphorylation process, participating in more than 90% of intracellular ATP synthesis. By supplementing NAD+, NMN can significantly improve the efficiency of the mitochondrial respiratory chain, alleviate chronic fatigue, slow metabolism, decreased physical strength and other problems caused by mitochondrial dysfunction. At the same time, it regulates sugar, lipid and amino acid metabolism, maintains the body’s energy balance, and provides sufficient power for cellular activities.

**Technical Application**: It is suitable for health management programs for office workers and middle-aged and elderly groups, which can relieve fatigue and improve energy. In the field of sports nutrition, it can assist in post-exercise recovery and reduce muscle damage. In the field of scientific research, it is used for experimental research related to cellular metabolism and mitochondrial function, with high data reproducibility.

3.Gene Repair and Neuroprotection: Multi-Dimensional Protection of Cellular Health

NAD+ is the only substrate for core enzymes such as Sirtuins deacetylases and PARP polymerases. By increasing NAD+ levels, NMN can activate the Sirtuins family (SIRT1-7), regulate telomere maintenance and cell apoptosis, promote DNA damage repair, and reduce the risk of cellular mutations. At the same time, NMN can improve neural synaptic plasticity, promote neurotransmitter synthesis, protect neurons from damage, alleviate cognitive decline, and has important value for the research of neurodegenerative diseases.

**Technical Application**: In the field of scientific research, it is used for the mechanism research of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. In high-end health management, it can be used for conditioning programs related to cognitive function improvement and neuroprotection. In formulation R&D, it can develop special raw material formulas for neural health.

4.Technical Application Scenarios in Segmented Fields

IV.Our Core Technical Advantages (Differentiated Competitiveness)

1.Full Enzymatic Synthesis Process: Pure, Efficient and Industry-Leading

We adopt multi-enzyme synergistic catalysis technology expressed by recombinant Escherichia coli, using nicotinamide (NAM), ribose and ATP as substrates, and directionally synthesize β-NMN through catalysis by immobilized enzymes (RK, PPM, NRK). The whole process is free of heavy metal catalysts and organic solvent residues, completely solving the pain points of low purity, many impurities and high environmental pressure of traditional chemical synthesis methods. It is currently the recognized high-end production technology route in the industry.

Core Advantages: The biological activity retention rate is ≥98%, the β-isomer purity is ≥99.0%, and the purity fluctuation between batches is ≤0.2%; the reaction conditions are mild (around 25℃), the wastewater discharge is reduced by 70% compared with chemical synthesis methods, which is in line with green manufacturing standards; automated and continuous production is realized, with an annual output of more than 50 tons, which can meet the needs of large-scale bulk procurement. At the same time, through technical optimization, the production cost is reduced by 40% compared with traditional enzymatic methods, with significant cost performance advantages.

2.Multi-Stage Purification Technology: Ultimate Purity and Compliance

We have built a three-stage purification system of “Membrane Separation + Preparative HPLC + Low-Temperature Recrystallization” to screen impurities layer by layer, ensuring that the product purity is ≥99.5%, and completely removing isomers, trace by-products and endotoxins, which is far higher than the industry average level. It fully complies with USP/EP pharmacopoeial standards and relevant domestic specifications, and can be directly used in medical and scientific research scenarios. Each batch of products can pass the verification of authoritative third-party detection.

Purification Process: ① Membrane Separation Purification: Use nanofiltration membrane to intercept macromolecular impurities and salts, and remove small molecular metabolites; ② HPLC Refinement: Use preparative C18 column to accurately separate β-isomer, α-isomer and trace impurities; ③ Low-Temperature Crystallization: Recrystallize in a controlled environment of -5℃ to improve crystal purity and stability, reduce water content, and extend product shelf life.

3.Strict Quality Control and Stability Technology: Full-Link Quality Assurance

• Endotoxin Control: Production is carried out in a Class 10,000 clean workshop, and a dual endotoxin removal process of activated carbon adsorption + ultrafiltration is adopted to ensure that the endotoxin content meets the medical grade standard (≤0.005 EU/mg), adapting to the sterile requirements of IV infusion and eliminating clinical safety risks;
• Stability Enhancement: Vacuum freeze-drying pretreatment and nitrogen-filled packaging technology are adopted to minimize the risk of product oxidation and moisture absorption. The purity decline rate is <0.3% after 36 months of storage at -20℃, and high activity can still be maintained after 24 months of storage at 4℃, solving the industry problem of NMN’s easy oxidation and poor stability;
• Full-Process Traceability: A full-link quality traceability system is established from raw material procurement, enzymatic synthesis, purification and refinement, finished product detection to warehousing and delivery. Each batch of products is accompanied by an authoritative third-party COA quality inspection report, covering 15 core indicators such as purity, isomer content, endotoxin and heavy metals, and the quality is traceable and controllable.

4.Customized Technical Services: One-Stop Adaptation to Needs

• Product Customization: Supports purity customization (99.5%-99.9%) and endotoxin index customization (≤0.2EU/mg to ≤0.01 EU/mg), adapting to different scenarios such as scientific research, medical treatment and health care; adds diversified density and particle size powder customization, which can accurately adjust powder density (0.3-0.8 g/cm³) and particle size (100-800 mesh) according to customer needs, adapting to different formulation process requirements; at the same time, provides sustained-release pellet customization services, which can customize pellet particle size (0.5-2.0 mm) and sustained-release period (4-24 hours), adapting to oral sustained-release preparations, long-acting infusion preparations and other scenarios; packaging customization can provide sterile inner bags and customized labels to meet customers’ brand needs;
• Technical Docking: A professional R&D team provides one-to-one technical support, answering questions related to NMN enzymatic process, formulation, dissolution method, aseptic filling, etc., and assisting customers in completing dosage form R&D and compliance filing;
• Multi-Language Document Support: Provides multi-language versions of the “Product Technical Manual” in Chinese, English, etc., including COA reports, detection methods, storage specifications, application guidelines, etc., to support compliant promotion in overseas markets and adapt to the needs of global markets such as the European Union and North America.

V.Compliance and Quality Assurance System

1.Authoritative Qualification Certification

The products have passed dual domestic and international compliance certifications, with complete qualifications and global compliant circulation, including:

• Quality Management System Certification: ISO9001:2015 Quality Management System
• Pharmacopoeial and Compliance Standards: Complies with USP/EP pharmacopoeial standards and the requirements of the Chinese Pharmacopoeia (2020 Edition, Volume IV）;
• International Qualification Certification: FDA Registration and Filing, HALAL Certification;
• Detection Qualification: Cooperates with authoritative third-party detection institutions, and each batch of products can issue a compliant COA quality inspection report with true and traceable detection data.

2.Full-Process Quality Control

Strictly follows GMP production specifications, equipped with high-end detection equipment such as LC-MS/MS, HPLC, Tachypleus Amebocyte Lysate Detector and Atomic Absorption Spectrophotometer, realizing full-item detection from raw materials to finished products, and preventing unqualified products from leaving the factory:

• Raw Material Control: Select high-quality raw material suppliers, conduct strict detection on core raw materials such as nicotinamide and ribose, ensure that the raw material purity is ≥99.0%, and free of heavy metals and microbial contamination;
• Production Control: The whole production process is carried out in a Class 10,000 clean workshop, strictly controlling temperature, humidity and sterile environment to avoid pollution during production;
• Finished Product Control: Each batch of finished products is tested for 15 core indicators such as purity, isomer content, endotoxin, heavy metals and microorganisms, and can only leave the factory after passing the test to ensure consistent quality between batches.